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Membrane association of viral proteins in virus-infected cells. MDCK and CK/BM2 cells were infected with rg-B/Y wt (A), BM2stop (B), and BM2Δ51-80 (C) viruses. At 11 hpi, infected cells were lysed and postnuclear fractions were subjected to equilibrium centrifugation as described in Materials and Methods. Viral proteins in all fractions collected from the top (T) to the bottom (B) were analyzed by Western blotting with anti-B/Yamagata and anti-BM2 antibodies.
