FIG. 1.

Characterization of the naive and memory P14 transgenic CD8 T cells used for subsequent gene expression analysis. (A) Splenocytes from naive P14 transgenic or LCMV-immune P14 chimeric mice were isolated and stained with MHC class I tetramer specific for the LCMV GP33-41 epitope, anti-CD8, anti-Thy1.1, anti-CD44, and anti-CD62L antibodies and then analyzed by flow cytometry. Thy1.1, CD44, and CD62L expression is shown for tetramer and CD8 double-positive cells. (B) Thy 1.1 and CD8 double-positive transgenic T cells were sorted to >94% purity prior to RNA isolation. RNA was isolated from cells sorted as indicated here and was analyzed as described in the legend to Fig. 2.