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Activation state of transgenic cells following peptide stimulation. P14 transgenic T cells were labeled with CFSE, stimulated for 24 h with 1 μg of GP33-41 peptide/ml, stained with Hoechst 33342 and anti-CD8, anti-Thy1.1, and anti-CD69 antibodies, and then analyzed by flow cytometry. CD8 Thy1.1 double-positive cells are shown in each histogram (gates not shown).
