TABLE 3.

Sequences of PCR and annealing oligonuleotides used to make in vitro RNA synthesis plasmids

Oligonucleotide	Sequence (5′→3′)
T7S	AATTCTAATACGACTCACTATAGGGGTAC
T7A	CCCTATAGTGAGTCGTATTAG
T7PKS	CGTCGACACGCGTGGATCCA
T7PKAS	AGCTTGGATCCACGCGTGTCGACGGTAC
AdenoS	GGTACCAACAAAAGCTTGCATGCCTG
AdenoAS	GTCGACCTGTGGAAAAAAAAGGGACAGGATA
16L1-42wtS	CAGATGTCTCTTTGGCTGCCTAGTGAGGCCACTGTCTACTTGCCTA
16L1-42wtAS	AGCTTAGGCAAGTAGACAGTGGCCTCACTAGGCAGCCAAAGAGACATCTGGTAC
16L1-42mutS	CAGATGAGCCTGTGGCTGCCCAGCGAGGCCACCGTGTACCTGCCCA
16L1-42mutAS	AGCTTGGGCAGGTACACGGTGGCCTCGCTGGGCAGCCACAGGCTCATCTGGTAC
16L1(45)S	GTCGACGCGCGCGTCCCAGTATCTAAGGTTG
16L1(177,BamHI,HindIII)AS	AAGCTTGGATCCTTTGTTATTGTTAGG
16L1M(45)S	TCTAGAGCGCGCCGTGCCCGTGAGCAAGG
16L1M(177,BamHI,HindIII)AS	AAGCTTGGATCCCTTGTTGTTGTTAGGCTTC