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. 2016 Oct 5;119(1):13–26. doi: 10.1093/aob/mcw187

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© The Author 2016. Published by Oxford University Press on behalf of the Annals of Botany Company.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — (A) Southern blot hybridization of genomic DNA from several B. napus cultivars. In the left panel the blot was hybridized with the 26S probe. After stripping the blot was rehybridized with the A-genome-specific IGS probe (B). ‘C’, C-genome bands; ‘A’, A-genome bands; ‘A*’ indicates an IGS family amplified in a subset of B. napus cultivars. (C) Example of a Southern hybridization of the C-genome-specific IGS probe. Note strong hybridization of the probe to ‘Yudal’ DNA. (D) The radioactivity of bands was quantified using a Phosphorimager and homeologue gene number expressed as a proportion of C-genome rDNA to total rDNA. Numbers below indicate two major chloroplast haplotypes identified by Cifuentes et al. (2010). In the same study, the B. napus cultivars were divided into groups with low (–), intermediate (±) and high (+) frequency of homeologue chromosome pairing in meiosis (symbols towards the bottom).