FIG. 2.

Repopulation of donor CD34⁺ cells post-re-engraftment in the Thy/Liv implants of irradiated secondary SCID-hu recipients. (A) Reconstitution of exogenous HLA-B27⁺ CD34⁺ cells derived from the Thy/Liv implants of primary recipient SCID-hu animals following re-engraftment into the implants of irradiated secondary recipients endogenous for HLA-B8⁺ cells. (B) Backgating analysis of the HLA-B27⁺ and HLA-B27⁻/HLA-B8⁻ cell populations for c-Mpl and CD34 expression at 3 weeks post-re-engraftment. Both phenotypic and functional reconstitution following re-engraftment into irradiated secondary recipients is about 70 to 80%, as assessed by the proportion of c-Mpl⁺ CD34⁺ subset cells shown in this figure, and overall multilineage CFA (shown in Fig. 3), respectively, at 3 weeks post-re-engraftment.