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. 2004 Oct;78(20):11121–11129. doi: 10.1128/JVI.78.20.11121-11129.2004

FIG. 2.

FIG. 2.

Genetic analysis of wild-type KSHV BAC36 and mutant BAC36-ΔLNA. (A) Diagnostic PCR for the differential detection of BAC36 and BAC36-ΔLNA. (B) Restriction analysis of BAC36 and BAC36-ΔLNA by HindIII digestion. The 11.6-kb band in BAC36 was replaced by two bands of 7.6 and 5.2 kb in BAC36-ΔLNA because of the presence of a HindIII site in the transposon sequence. (C) Southern blot hybridization with a 578-bp DNA fragment from the LNA N-terminal domain as a probe. (D) Southern blot hybridization with Kanr DNA as a probe. M, molecular weight marker.