FIG. 3.

Infection with S protein-pseudotyped retrovirus is less efficient in cells expressing rodent ACE2 than in those expressing human ACE2. (A) An aliquot of cells analyzed as shown in Fig. 1B was incubated with S protein-pseudotyped SIV-GFP. After 48 h, the cells were harvested and analyzed by flow cytometry for GFP expression. GFP levels indicating infection (dark gray) are compared with anti-Myc antibody (light gray) and S1-Ig (medium gray) recognition in the same cells. (B) Same experiment as shown in panel A except that cells analyzed as shown in Fig. 1A were infected with SIV-GFP. (C) Typical image of GFP expression of cells transfected and infected as shown in panel B. The figure is representative of two similar experiments with similar results.