Fig 3. Increases in osmolarity in glucose media promote cyanide-resistance respiration.
(A) Cyanide-resistant respiration in cells grown in YPD. Cells (1 mg.mL-1) were incubated in respiration buffer. To induced Aox-dependent respiration, 25 mM glucose and 0.5 mM potassium cyanide (KCN) were included in the incubation media. (B-D) Densitometry analysis of the expression of the Aox (Panel B) and cytochrome c oxidase subunit III (CoxIII, Panel C) in glucose media in the presence of 0.6 M NaCl, 1.2 M sorbitol or 0.6 M KCl. (D) Representative blots of Aox and CoxIII expression.