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. 2017 Jan 6;12(1):e0169621. doi: 10.1371/journal.pone.0169621

Fig 5. Increased osmolarity decreases the activity of the cytochrome pathways.

Fig 5

A. Oxygen consumption in logarithmic phase cells. Cells (1 mg mL-1) were grown in YPD media, harvested, washed and suspended in the indicated incubation media. White bars, respiration in YPD media. Light gray bars, cytochrome-dependent respiration in the presence of SHAM. Dark gray bars, Aox-dependent respiration in the presence of cyanide. B. Oxygen consumption in cells in the logarithmic phase. Cells (1 mg mL-1) were grown in YPD plus 0.6 M NaCl, harvested, washed and suspended in different incubation media. Bar code is the same as in Fig 5A. Incubation media were composed of 1% yeast extract and 2% peptone without a carbon source (YP). YP base was supplemented with sorbitol (1.2 M) NaCl (0.6 M) or KCl (0.6M). Respiration was induced by the addition of 25 mM glucose. Additions: 3 mM SHAM, 0.5 mM potassium cyanide. * p < 0.05 respect to Aox (dark bars) or cytochrome-dependent (light bars)respiration.