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. 2017 Jan 6;12(1):e0169621. doi: 10.1371/journal.pone.0169621

Fig 7. Alternative oxidase activity is necessary for growth in high osmolarity media.

Fig 7

(A) Cells were grown in YPD media with and without NaCl, KCl or sorbitol (white bars). Some plates were supplemented with 3 mM SHAM (gray bars) to determine the effect of Aox on hyperosmotic media. Samples were spread on YPD plates at 28°C and colonies were counted after 2 days. (B) Representative plates of serially diluted samples to show the effect of 3 mM SHAM on cell grown in the presence of the indicated osmolites. Ctrl, YPD media, Na+, YPD plus 0.6 M NaCl, S, YPD, YPD media plus 1.2 M sorbitol, K+, YPD media plus 0.6 M KCl. (C-D) Compatible solute production in the cells grown in the presence of the indicated osmolites. Cells grown in the indicated media were lysed and extracts assayed for trehalose (C) and glycerol (D) content. * p < 0.05 respect to control cells.