Fig 1. Iron mediated alterations in the levels of M. tuberculosis ferritins.

M. tuberculosis H37Rv strain was grown in the minimal media either without iron (-) or with increasing iron concentrations (ranging from 10 μM to 250 μM). 50 μg of the lysate protein was subjected to 15% SDS-PAGE followed by immunoblotting by using antibodies against (a) BfrB and (b) BfrA of M. tuberculosis. For loading control, the same lysates were also immunoblotted by using antibodies against SigA. The immunoblot shown is representative of one experiment, however, the same pattern was observed across three experiments. P: Purified protein (positive control).