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. 2004 Oct;78(20):11172–11186. doi: 10.1128/JVI.78.20.11172-11186.2004

FIG. 1.

FIG. 1.

Detection of viral DNA and nascent transcription centers in CaSki and SiHa cervical cancer cell lines. (A and E) Detection of HPV-16 DNA (Cy3; red) in SiHa cells (A) or CaSki cells (E) with nick-translated probes in permeabilized interphase cells. (B and C) Simultaneous in situ detection in a single interphase SiHa cell of nascent HPV-16 RNA (FITC) (B) and HPV-16 DNA (Cy3) (C) by sequential hybridization with a nick-translated genomic probe. (D) Interphase chromosome analysis of SiHa cells for nascent HPV transcripts (FITC) and the chromosome 13 territory (Cy3). (F to H) Simultaneous in situ detection in a single interphase CaSki cell of nascent HPV-16 RNA (FITC) (F) and viral DNA (Cy3) (G) by sequential hybridization with a nick-translated genomic probe. (H) Merged image of panels F and G. Images were captured with a ×40 (A and E) or ×100 (B to D and F to H) objective lens but enlarged to different extent to display several cells (A and E) to show reproducibility or for a single cell for clarity (B to D and F to H). In all panels, nuclear DNA was stained with DAPI (blue).