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. 2004 Oct;78(20):11172–11186. doi: 10.1128/JVI.78.20.11172-11186.2004

FIG. 3.

FIG. 3.

Localization and number of transcriptionally active HPV centers in immortalized human foreskin keratinocytes. (A to F) HPV RNA centers (FITC, green) during passage relative to nucleoli, which was revealed by antibody to nucleolin or C23 (TRITC, red). Nascent viral RNAs were detected by in situ hybridization with DNA probes in FK16A at passages 23 and 82 (A and B), FK16B at passages 27 and 92 (C and D), FK18A at passages 23 and 114 (E and F), or FK18B at passage 115 (G, H, and I). Nuclear DNA was stained with DAPI. (G to I) Localization of HPV RNA center (FITC) relative to HPV DNA in the FK18B. (H and I) Enlargement of two of the three cells in panel G (boxes). All images were acquired with a ×100 objective lens but were enlarged in panels H and I.