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Impact of wt and pp65 mutant CMV infection on NF-κB localization. Shown are immunofluorescence assays of NF-κB localization in HFs infected (MOI of 4) with wt virus at 4 (A and D) or 8 (G and I) hpi or with pp65 mutant virus (mut) at 4 (B and E) or 8 (H and J) hpi compared to mock-infected cells at 4 (C and F) hpi. Texas Red-conjugated secondary antibody was used to generate the results shown in panels G through J. Overlays of Hoechst 44432-positive nuclei are shown (merge).
