Extended Data Figure 3. Reduced XRCC1 recruitment into damaged chromatin in XRCC1 patient cells.

a, XRCC1 recruitment into chromatin was compared in the indicated cell lines by ScanR high content imaging before and 10 min after treatment with 1 mM H₂O₂. Cells were pre-extracted with detergent prior to fixation and immunostaining. Representative images of the ScanR (Olympus) data used for the quantification in Fig. 2d are shown. b, XRCC1 recruitment into chromatin using high resolution Zeiss microscope was compared in the indicated cell lines after treatment 45 min with 30 μM CPT. Cells were pre-extracted with detergent prior to fixation and immunostaining as above. Scale bars in images are 10 μm.