Extended Data Figure 5. Elevated ADP-ribosylation in XRCC1^-/- RPE-1 cells, XRCC1 patient fibroblasts, and PNKP patient fibroblasts.

a, ADP-ribosylated proteins were detected in cell extracts from wild type RPE-1 cells (“WT”), XRCC1^-/- RPE-1 cells, wild type 1BR fibroblasts, XRCC1 patient fibroblasts (“X1 patient”), and PNKP patient fibroblasts treated as in Fig. 4a by Western blotting and Anti-pan-ADP-ribose binding reagent. b, Levels of ADP-ribosylation in 1BR wild type, XRCC1 patient, and PNKP patient fibroblasts measured before and after CPT treatment by indirect immunofluorescence as indicated in Fig. 4b. Representative ScanR images are shown.