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. Author manuscript; available in PMC: 2017 Jun 21.
Published in final edited form as: Nature. 2016 Dec 21;541(7635):87–91. doi: 10.1038/nature20790

Extended Data Figure 6. Elevated ADP-ribose levels in CPT-treated XRCC1-/- RPE-1 cells are PARP1 dependent.

Extended Data Figure 6

a, Representative ScanR images of wild type (“WT”), XRCC1-/-, PARP1-/-, and XRCC1-/-/PARP1-/- RPE-1 cells before and after 30 μM CPT treatment stained by indirect immunofluorescence for XRCC1, PARP1, DAPI or ADP-ribose. b, Quantification of ADP-ribose intensity in the nucleus from >3500 cells per sample from a (data from single experiment). c, Western blot showing XRCC1 and PARP1 protein levels in the indicated RPE-1 cell lines.

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