Fig. 2.

IL37tg mice show attenuated sleep responses to IL-1β and LPS. WT mice (white symbols) and IL37tg mice (black symbols) were IP injected with saline (0.2 ml) just prior to dark onset and EEG/EMG was recorded for 24 h. Mice (n=7/strain) then received 180 ng of IL-1β [A, B, C] or 500 ng lipopolysaccharide (LPS; n=8/strain; D, E, F) and recording continued. Duration in state data are presented as differences from saline in NREMS [A,D] and REMS [B, E] averaged over 12 h periods corresponding to dark and light periods. EEG power spectra (1–20 Hz) from artifact-free NREMS-scored epochs over the first 2 h post-injection (ZT12-14) are depicted [C, F; WT top upper panels, IL37tg bottom panels]. Asterisks indicate within strain, treatment differences (* = p<0.05; ** = p<0.01) and pound signs indicate differences between strains (# = p<0.05; ## = p<0.01; gray fill indicates dark period).