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Potential antimicrobial effect of the functionalized multilayer films, according to the number of peptide layers. No effect could be observed on films constructed with a defensin solution of 5, 10, 50, or 100 μM (data not shown). Nevertheless, the increase in the number of layers of defensin (in a 10 μM solution) increases the antimicrobial effect of the film up to 98.75% with 10 layers of defensin. *, P > 0.1; ***, P > 0.001; CTRL, control.
