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. 2017 Jan 6;12(1):e0169587. doi: 10.1371/journal.pone.0169587

Fig 4. DUB3 mediates ITCH, LATS1/2 and AMOT proteins to regulate Hippo activity.

Fig 4

(A) A schematic view of DUB3-mediated regulation of Hippo signaling. DUB3 de-ubiquitylates ITCH, LATS1/2 and AMOT to promote their stability. In the presence of stabilized AMOT, ITCH promotes YAP degradation. (B) Luciferase reporter assays showing the effects of ITCH overexpression on YAP/TAZ activity. HEK293T cells were transfected to express the luciferase reporters together with a control or ITCH expression vector in the presence of a mixture of siRNAs targeting AMOT, AMOTL1 and AMOTL2, a mixture siRNAs targeting LATS1 and LATS2 or a scrambled siRNA control. Data represent the average of three independent transfection experiments ± SD. (C) Luciferase reporter assays showing the effects of DUB3 siRNAs on YAP/TAZ activity. HEK293T cells were transfected to express the luciferase reporters together with a control or DUB3 siRNA in the presence of ITCH siRNA, a mixture of siRNAs targeting ITCH and NEDD4 or a scrambled siRNA control. Data represent the average of three independent transfection experiments ± SD.