E2-treated CSCs empower their migratory activity in vivo. Intrasplenic injection in NOD/SCID female mice of 3 ×105 luciferase (LUC)/GFP-transduced cells, untreated or treated with 1 nmol l−1 E2 for 24 h (magnitude 10×, EVOS™, Thermo Fischer, Waltham, Massachussetts). Analysis of migratory capacity at time: 0 min; 30 min; 5 weeks. Biospace.