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. Author manuscript; available in PMC: 2017 Jan 6.
Published in final edited form as: Mol Microbiol. 2009 Oct 15;74(5):1031–1043. doi: 10.1111/j.1365-2958.2009.06915.x

Fig. 3.

Fig. 3

HPLC profiles of whole-cell biotransformations of cholesterol by cell cultures of (A) R. rhodochrous strain RG32 showing the formation of 1,4-androstadiene-3,17-dione (ADD) and 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid (Δ1,4-BNC) via selective sterol side-chain degradation, (B) mutant strain RG32Ωcyp125 and (C) cyp125DSM43269 complemented mutant strain RG32Ωcyp125. HPLC profiles of whole-cell biotransformations of 5-cholenic acid-3β-ol (D) and 5-cholestene-26-oic acid-3β-ol (E) by cell cultures of R. rhodochrous mutant strain RG32Ωcyp125 are also shown. Profiles of authentic ADD (50 μM, F) and Δ1,4-BNC (G), obtained by incubating authentic 3-oxo-23,24-bisnorchol-4-en-22-oic acid (50 μM, Δ4-BNC) with purified Δ1-KSTD1 (Knol et al., 2008), are included as reference samples. Insets: GC profiles showing the accumulation of 4-cholestene-3-one (2), 1,4-cholestadiene-3-one (3) and 5α-cholestane-3-one (4) from cholesterol (1) by R. rhodochrous mutant strain RG32Ωcyp125, but not strain RG32.