TABLE 2.

Nontoxic concentrations of the candidate microbicide and placebo formulations in epithelial cell lines and primary immune cells^a

Product	Nontoxic concn. in epithelial cell lines				Nontoxic concn. in primary immune cells
	Caco-2	SW837	HEC-1-A	ME-180	PBMCs	MΦs
CAP	0.52	0.52	0.52	0.52	0.26	0.26
Carraguardb	0.3	0.3	0.3	0.3	0.3	0.6
Methylcellulose	0.5	0.5	0.5	0.5	0.25	0.5
PRO 2000 4%	0.16	0.16	0.16	0.16	0.16	0.2
PRO 2000 0.5%	0.02	0.02	0.02	0.02	0.05	0.05
PRO 2000 placebo	0.01	0.01	0.04	0.01	0.04	0.05
KY-N9	0.002	0.002	0.002	0.002	0.002	0.002
KY Jelly	0.01	0.01	0.01	0.01	0.01	0.01
SPL7013 5%	0.2	NDc	0.2	ND	0.1	0.1
SPL7013 placebo	0.2	ND	0.2	ND	0.1	0.2
UC781 1%	0.02	0.1	0.1	0.1	0.2	0.2
UC781 0.1%	0.002	0.01	0.01	0.01	0.02	0.02
UC781 placebo	0.02	0.1	0.1	0.1	0.2	0.2
Vena Gel	0.001	0.001	0.001	0.001	0.001	0.001
Vena Gel placebo	0.65	0.65	0.65	0.65	0.325	0.0325

^aThe epithelial cell lines and primary immune cells were cultured with concentrations of products or placebos for 24 h. After washing, culture viability was determined by using the Cell-Titer-Glo assay according to the manufacturer's instructions. The values given are the ratios of the concentrations of the products or placebos that resulted in ≥60% culture viability to the concentrations of the original formulations.

^bNontoxic concentration of Carraguard was determined by trypan blue exclusion.

^cND, not determined.