Table 1.
Primer-probe set name | Forward primer sequence | Reverse primer sequence | Probe sequence |
---|---|---|---|
Primer/FAM-ZEN COM-DEL | 5′-GGC TCA GGC GTT TGT GTA TGAT-3′ | 5′-TAT TAA ACA CAA ACT ACC ACC TAC C-3′ | 5′-FAM/ACC ATT GGC/ZEN/AGC CTA G/IBFQ-3′ |
Primer/HEX-ZEN RPP30 | 5′-GAT TTG GAC CTG CGA GCG-3′ | 5′-GCG GCT GTC TCC ACA AGT-3′ | 5′-HEX/CT GAC CTG A/ZEN/A GGC TCT/IBFQ-3′ |
Primer/FAM-ZEN ND2 | 5′-CTT CTG TGG AAC GAG GGT TTA T-3′ | 5′-CCC GTC ATC TAC TCT ACC ATC T-3′ | 5′-FAM/ACA CTC ATC/ZEN/ACA GCG CTA AGC TCG/IBFQ-3′ |
Sequences for each ddPCR primer-probe set used.