Figure 2. SEM images show that AC domain inhibits biofilm formation on glass coverslips.

B. pertussis was grown in 24 well plates with inverted glass coverslips so that biofilm formation could occur at the air liquid interface. At 96 hours, the coverslips were fixed in 4% paraformaldehyde and prepared for SEM imaging using a Zeiss Sigma VP HD field emission Scanning Electron Microscope at the University of Virginia Microscopy Core. Representative images were chosen from four experimental replicates. (A) WT BP338 (15000 X). (B) Bvg(−) BP347 B. pertussis (5000 X). (C) WT BP338 + 10 ng mL⁻¹ AC domain (5000 X).