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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Mol Microbiol. 2016 Nov 3;103(2):214–228. doi: 10.1111/mmi.13551

Figure 6. The AC domain and ACT inhibit MCD antibody binding to FHA.

Figure 6

In an ELISA binding assay, plates were coated with FHA and an anti-MCD antibody was used as a detection reagent. ACT, AC domain and ACTΔAC, added at a range of µg mL−1 concentrations, were added prior to the addition of the anti-MCD antibody to determine if AC domain binding interfered with MCD antibody detection. In addition to these conditions, 10 µM CaM was incubated with the various concentrations of ACT or AC domain for 15 minutes prior to addition of ACT to the wells. All values were normalized to the control (OD450 0.284) using GraphPad Prism6 software. Data expressed as the mean ± standard deviations, compiled from 3 experiments run in triplicate ** = p <0.01 and **** = p<0.0001 compared to control.