TABLE 2.
Phenotypes and mechanisms of antibiotic resistance detected in the 17 multiresistant E. coli strains of this study
| E. coli strain (origin)a | Phenotype of resistanceb | Mechanisms of resistance
|
|||
|---|---|---|---|---|---|
| Resistance genes detected | Position(s) of amino acid change(s) in:
|
CATc | |||
| GyrA | ParC | ||||
| Co1 (F) | Nal Cip Amp Kan Str Rif Tet Chl Tmp Smx | blaTEM1b, aphA1, aphA2, aadA1, tetB, dfrA1, sul2 | 83 + 87 | 80 | |
| Co19 (F) | Nal Amp Kan Str Rif Tet Chl Tmp Smx | blaTEM1b, aphA2, aadA1, tetB, dfrA1, sul2 | 83 | ||
| Co45 (F) | Nal Cip Amp Amcd Kan Str Rif Tet Chl Tmp Smx | blaTEM1b, aphA2, aadA1, tetA, dfrA1, sul2 | 83 + 87 | 80 | |
| Co53 (F) | Nal Amp Amcd Kan Str Rif Tet Chl Smx | blaTEM1b, aphA2, tetB, sul2 | 83 | ||
| Co71 (B) | Nal Cip Amp Kan Str Rif Tet Chl Tmp Smx | blaTEM1b, aphA1, aadA5, tetB, dfrA17, sul1, sul2 | 83 + 87 | 80 | + |
| Co80 (B) | Nal Amp Gen Tobd Kan Str Rif Tet Chl Smx | blaTEM1b, aac(3)-II, aphA2, aadA1, tetB, sul1, sul2 | 83 | + | |
| Co82 (B) | Nal Cip Amp Kan Str Rif Tet Chl Tmp Smx | blaTEM1b, aphA1, aadA5, tetB, dfrA17, sul1, sul2 | 83 + 87 | 80 | + |
| Co110 (B) | Nal Amp Amcd Str Rif Tet Chl Tmp Smx | blaTEM1a, aadA1, tetA, cmlA, dfrA1, sul1, sul3 | 83 | ||
| Co125 (P) | Nal Cip Amp Str Rif Tet Chl Tmp Smx | blaTEM1b, aadA1, aadA2, tetA, cmlA, dfrA12, dfrA1-like, sul3 | 83 + 87 | 80 | |
| Co279 (P) | Nal Amp Amcd Str Rif Tet Chl Tmp Smx | blaTEM1b, aadA1, aadA2, tetB, cmlA, dfrA12, sul3 | 83 | ||
| Co177 (D) | Nal Amp Amcd Str Rif Tet Chl Tmp Smx | blaTEM1b, aadA1, tetA, dfrA1, sul1, sul2 | 83 | + | |
| Co201 (D) | Nal Amp Amcd Str Rif Tet Chl Tmp Smx | blaTEM1b, aadA1, tetA, dfrA1, sul1, sul2 | 83 | + | |
| Co227 (H) | Nal Amp Amcd Gen Apr Tob Str Rif Tet Chl Tmp Smx | blaTEM1b, aac(3)-IV, aadA1, aadA2, tetA, cmlA, dfrA1, dfrA12, sul1, sul2, sul3 | 83 | ||
| Co228 (H) | Nal Amp Amcd Gen Apr Tob Kan Str Rif Tet Chl Tmp Smx | blaTEM1a, aac(3)-IV, aphA2, aadA2, tetA, cmlA, dfrA12, sul1, sul3 | 83 | ||
| Co232 (H) | Nal Cip Amp Str Rif Tet Chl Tmp Smx | blaTEM1b, aadA1, tetA, dfrA1, sul1, sul2 | 83 + 87 | 80 + 84 | + |
| Co354 (H) | Nal Cip Amp Gen Apr Tob Str Rif Tet Chld Tmp Smx | blaTEM1b, aac(3)-IV, aadA1, aadA2, tetA, dfrA1, dfrA12, sul1, sul3 | 83 + 87 | 80 | |
| Co356 (H) | Nal Cip Amp Str Rif Tet Chl Tmp Smx | blaTEM1b, aadA5, tetB, dfrA17, sul1, sul2 | 83 + 84 | 80 + 108 | + |
a
F, food; B, broiler; P, pig; D, dog; H, human.
b
Nal, nalidixic acid; Cip, ciprofloxacin; Amp, ampicillin; Amc, amoxicillin-clavulanic acid; Gen, gentamicin; Apr, apramycin; Tob, tobramycin; Kan, kanamycin; Str, streptomycin; Rif, rifampin; Tet, tetracycline; Chl, chloramphenicol; Tmp, trimethoprim; Smx, sulfamethoxazole.
c
CAT, chloramphenicol-acetyl-transferase enzymatic activity. +, CAT was detected for the strain indicated.
d
Resistance to the drug indicated is in the intermediate category according to NCCLS standards for the corresponding strain.