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. Author manuscript; available in PMC: 2018 Jan 5.
Published in final edited form as: Mol Cell. 2016 Dec 22;65(1):168–175. doi: 10.1016/j.molcel.2016.11.031

Figure 3. hCas9 has increased interference efficiency against NAG- but not NGG-adjacent targets.

Figure 3

See also Figure S3.

(A) Growth curves of cultures infected with ϕNM4γ4 harboring the wtCas9 or hCas9 (but not Cas1, Cas2 and Csn2) programmed to target either NAG- or NGG-flanked viral sequences.

(B) Phage propagation, measured in pfu/ml, of the bacteria presented in A.

(C) Cleavage of radiolabeled dsDNA targets flanked by either NGG or NAG PAMs, by wtCas9 or hCas9.

(D) Quantification of the cleavage results shown in C. Data are represented as mean ± SD of 3 representative biological replicates.