TABLE 2.

S. cerevisiae strains for which changes in CAS MICs were greater than fourfold but for which the MICs of at least three of four additional antifungal compounds were those for the wild type

Gene name	Gene function	Median MIC (μg/ml)					A. fumigatus homolog	C. albicans homolog	E value for humans	Growth ratea
		CAS	AMB	CAL	5FC	FLC
Wild type		0.06	1	25	100	64				100 ± 12
SLG1	Cell wall and stress response	0.24	0.5	12.5	100	32	−b	+c	−	106 ± 6
BCK1	MAPkkk Rho1/PKC pathway	0.007	1	6.25	100	64	+	+	−	107 ± 2
FKS1	Glucan synthase	0.015	0.5	25	100	32	Fkspd	GSCId	NFe	98 ± 8
SMI1/KNR4	Glucan biosynthesis	0.007	0.5	12.5	50	32	+	+	−	97 ± 18
CHS4/SKT5	Chitin synthase regulation	0.015	0.5	>100	100	64	+	CHS4	NF	97 ± 18
ERG5	Ergosterol biosynthesis	0.015	1	25	50	>128	+	+	−	114 ± 11
TPO1	Polyamine transport	0.007	1	12.5	200	32	+	+	NF	92 ± 12
ILM1	Unknown	0.007	0.5	12.5	50	64	NF	+	NF	83 ± 12

^aPercent growth compared to that of wild-type strain BY4741 ± standard deviation.

^b−, only a gene with a low level of similarity was found in a search with the BLAST program (E value, >10⁻²⁰).

^c+, a highly similar, homologous gene was found in a search with the BLAST program (E value, <10⁻²⁰).

^dGene name of the homolog in A. fumigatus or C. albicans.

^eNF, not found.