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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Mol Microbiol. 2016 Nov 2;103(2):253–268. doi: 10.1111/mmi.13554

Fig. 1. Increased Mn(II) uptake does not account for the Mn(II) sensitivity of an mntR null mutant.

Fig. 1

Disk diffusion assays were performed to monitor sensitivity to Mn(II). Mid-logarithmic phase cells (OD600 ~0.4) were plated on LB agar plates and overlaid with 10 μl of 100 mM MnCl2 on a filter paper disk. The zone of growth inhibition was measured after overnight growth. Data represent the mean ± SD for at least three biological replicates. Significant differences from WT were determined by ANOVA Dunnett-test as indicated: *, P < 0.05; ****, P < 0.0001.