Table 2.

Approaches to reach high synthesis yields

Approach	Lipases	Glycoside hydrolases
Reversed hydrolysis
Low water concentration	+++	+
High alcohol concentration	++	++
Enzyme selectivity	–	–
Transfer reaction
Low water concentration	+++	+
High alcohol concentration	+	++
Enzyme selectivity	+++	+ 1) +++2)

The degree of success of different approaches is estimated using the following scale: +++, excellent results obtained; ++, good results obtained; +, moderate success so far; –, this approach does not work; 1), can be improved for some glycoside hydrolases; 2) transglycosylases