Figure 3.

The effect of silibinin treatment against UVB-induced skin damage in SKH-1 p53^−/− mice. p53^−/− mice were exposed to UVB alone (90 mJ/cm²) with or without topical application of silibinin (9 mg in 200 µl acetone) either prior to UVB (Sb + UV) or immediately after UVB exposure (UV + Sb) 3 days per week. (A) Survival curve of p53^−/− mice after chronic UVB exposure (n = 5–6 per group). (B) Representative photographs of mild hyperplasia and AK observed in H&E stained sections. Average number of mild hyperplasia and AK (per 200× field) in various groups at the end of the study are presented as bar diagram as mean ± SEM (n = 4).