Figure 6.

The effect of silibinin treatment on UVB-induced inflammation in p53^+/+ and p53^+/− mouse skin after 8 weeks of UVB exposure. Mice were exposed to UVB with or without topical application of silibinin (9 mg in 200 µl acetone) prior to UVB (Sb + UV) or immediately after UVB exposure (UV + Sb) 5 days per week for 8 weeks as detailed in Materials and Methods. At the end, mice were killed and skin was collected and analyzed by IHC. (A) IHC staining for NF-κB p50, COX-2, iNOS, TNFα, IL-6 and IL-12. Representative photomicrographs are show at 400× magnification. (B) Relative percentage of stained cells or score for each IHC staining. The data shown in the bar diagram represents mean ± SEM of three samples. *P < 0.05 and **P < 0.001 versus respective UV irradiated group.