FIG. 2.

Expression of ERG11, ABC transporters CDR1 and PDH1, and ACT1 in laboratory-derived fluconazole-resistant mutants (R; F15 to F26), their parent 66032, and three additional azole-susceptible strains (S). (A) RNA was isolated from log-phase cultures, blotted to membranes, and hybridized to the indicated gene probes as described in Materials and Methods. (B) Histogram of ERG11, CDR1, and PDH1 gene expression in individual resistant isolates relative to their susceptible parent 66032 (R/S). Expression was quantified by scanning and normalized to ACT1. Bars (left to right) represent the resistant isolates shown in panel A (top to bottom, left to right).