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. 2004 Oct;48(10):3989–3995. doi: 10.1128/AAC.48.10.3989-3995.2004

FIG. 3.

FIG. 3.

Chromosomal location of tet(C) in the resistant strains. (A) PCR results using S45 genomic DNA (lanes 1 to 3) or R19 genomic DNA (lanes 4 to 6) as template. Lanes 1 and 4 show a product amplified using primers that link the inv-like gene to the 23S rRNA. Lanes 2 and 5 show amplified products linking the inv-like gene to dmpP. Lanes 3 and 6 are products amplified from repC, a gene within the genomic island, to dmpP. Note that a product is generated from R19 template when primers for repC and dmpP are used (lane 6), but no such product is produced in a parallel reaction using strain S45 as a template (lane 3). Molecular size standards are indicated (in kilobases) to the left of panel A. (B) Linkage map showing how tet(C) and flanking sequences are positioned between dmpP and the 23S rRNA gene in R19. The dashed lines indicate the genomic sequence between genes targeted by the amplification.