TABLE 2.
Oligonucleotide primers used for PCR and nucleotide sequence analysis
| Primer name | Description | Sequence (5′→3′) |
|---|---|---|
| CS43 | tet(C) | AGCACTGTCCGACCGCTTTG |
| CS47 | tet(C) | TCCTCGCCGAAAATGACCC |
| CS33 | tetR(C) | CAAGACCGCCGATGATGAGAG |
| CS38 | tetR(C) | CCAAGGGATGACGACGACTG |
| CS09 | orfB | TTTTGACGCTTCGTTGAGCAC |
| CS17 | orfB | AGTCGGTTATTGGTTGATAGCAGC |
| CS01 | 3′-inv-like | GACTATCGGTAGACAAGACTCGGC |
| CS08 | 3′-inv-like | GACTCTCAAGGGAAGATTCGCAC |
| CS02 | Intact inv-like | CGTTTCAGGAATACCCACTTCG |
| CS106 | Intact inv-like | ACACTTCAGGTTTTCGCCGTAG |
| CS105 | 5′-inv-like | ATCATTCGCAACAGGAGG |
| CS107 | 5′-inv-like | CTTCGCCTCTCTTCACAAC |
| CS109 | dmpP | TGGTCTCTATCCTTCGTGGG |
| CS111 | 23S rRNA | TTTGTGGTCCAGCACTTC |
| CS05 | 3′-inv-like | TGCCGTCAACAACAGATGC |
| CS108 | dmpP | TGGTGTGTCGGTTGTTCTG |
| CS101 | repC | TGGGAAGAAGCATCAACG |
| CS68 | mobD | GAGTTCTTCAAGCGTGCTATTC |
| CS77 | mobB | ATCATTGCCAACGCCGACGC |
| CS81 | mobA | CGGCATACAGACACTTTTCC |
| CS84 | mobA | GCAGCCATCATCAAGCAG |
| CS86 | mobA | TGCTCCTGCCGCTTGTCCTG |