Fig. 7.

Susceptibility of mo-DCs, LCs, and LCs/IFN-γ to various maturation signals. (A) Cells were harvested at day 6 and treated with LPS (100 ng/ml), NY-ESO-1 IC (10 μg/ml protein, 5 μg/ml specific mAb), or CD40L (1 μg/ml and enhancer) for 2 days, then stained for CD86, CD83, and HLA-DR expression and analyzed by FACS. (B) LCs were incubated with NY-ESO-1 IC (25 μg/ml protein and 5 μg/ml mAb preincubated for 30 min), protein alone (25 μg/ml) in the presence of LPS (100 ng/ml) and CD40L (1 μg/ml and enhancer) or not, or synthetic peptide p157-165 (10 μM) for 7 h at 37°C. ELISPOT was performed by using clone49. Error bars indicate standard deviation in duplicate wells. Results were representative of at least three independent reproducible experiments.