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. 2004 Sep 23;101(40):14539–14544. doi: 10.1073/pnas.0403174101

Fig. 1.

Fig. 1.

CTD phosphatase activity of AtCPL proteins. (A) Protein preparations. Aliquots of the nickel-agarose preparations of S. pombe Fcp1 (SpFcp1; 6 μg), NusA (8 μg), NusA-CPL1 (12 μg), and NusA-CPL2 (12 μg) were analyzed by SDS/PAGE. The polypeptides were visualized by staining with Coomassie blue dye. The positions and sizes (in kDa) of marker proteins are indicated on the left. The polypeptides corresponding to the full-length NusA-CPL fusion proteins are indicated by arrows. (B and C) CTD phosphatase activity. Reaction mixtures (25 μl) containing 50 mM Tris-acetate (pH 5.5), 10 mM MgCl2, either 22 μM CTD Ser-5-PO4 phosphopeptide (YSPTSPS)4 (containing 2.2 nmol of input Ser-PO4) or 25 μM CTD Ser-2-PO4 phosphopeptide (YSPTSPS)4 (containing 2.5 nmol of input Ser-PO4), and recombinant proteins as specified were incubated for 60 min at 37°C. Phosphate release is plotted as a function of input protein.