Table 1. Reciprocal cross analysis indicated synthetic lethality in fry2-1 cpl2-2 male gamete.

Cross					fry2-1 phenotype, genotype
Female		Male	Total	WT phenotype	CPL2	cpl2-2/+
fry2-1	×	fry2-1/+cpl2-2/+	112	76	36	0
fry2-1/+cpl2-2/+	×	fry2-1	62	39	12	11

Each F₁ seedling was first scored for fry2-1 phenotype by using RD29a-LUC expression after cold treatment (0°C, 48 h) and then after ABA treatment (100 μM, 3 h). Seedlings with fry2-1 phenotype were recovered, and their genomic DNA was extracted. The CPL2 genotype of fry2-1 F₁ seedlings was determined by detecting T-DNA insertion in CPL2 by using PCR.