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. 2004 Sep 22;101(40):14390–14395. doi: 10.1073/pnas.0405692101

Fig. 3.

Fig. 3.

Cleavage of proHp by recombinant C1r-LP. (A) Cells were transfected with vector expressing myc-His tagged C1r-LP or mock vector, and cells and media were analyzed by Western blotting with antibodies to the myc epitope. The molecular masses (in kilodaltons) of reference proteins are shown on the right. (B) Cells were transfected with vector expressing proHp, and either mock vector, or vector expressing either C1r-LP, or a C1r-LP S436A mutant (C1r-LP*). Subsequently, cell media were analyzed by Western blotting with antibodies to Hp (α-Hp) or to myc-tag (α-myc). (C) myc-His6-tagged proHp and C1r-LP were isolated as described in Materials and Methods. ProHp was mixed with C1r-LP or buffer only and incubated for 16 h at 37°C. Subsequently, proHp cleavage was analyzed by Western blotting.