Skip to main content
. 2016 Dec 28;11:365–374. doi: 10.1016/j.redox.2016.12.026

Fig. 4.

Fig. 4.

Young individuals demonstrate a stronger ability to degrade oxidatively damaged proteins by engaging chaperones and the proteasome system. (A) The relative expression of hsf-1, hsp60 and hsp70 in Day 1 and Day 12 worms treated with PQ was assessed by performing qRT-PCR. (B) Changes in hsp4 or hsp6 expression in response to challenge were measured in young and old hsp4pr::gfp or hsp6pr::gfp transgenic worms. (C, D) Changes in the expression of the chaperones HSC70, HSP70, HSP60 and HSP90 in p34 and p42 cells in response to PQ challenge were assessed by performing qRT-PCR and Western blotting. (E) Proteasome activity in young and old worms in response to PQ stress was measured in a fluorogenic peptide substrate assay. (F) Proteasome subunit levels (PMSE2, PMSB4 and PMSD8) in p34 and p42 cells at 24 h after PQ treatment were assessed by performing qRT-PCR. (G) Proteasome activity in young and senescent cells in response to PQ-induced stress was quantified using a fluorogenic peptide substrate assay. (H) LON levels in p34 and p42 cells at 12 h and 24 h after PQ treatment were assessed by performing qRT-PCR. The error bars indicate the SEM (n=3).