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. 2017 Jan 7;18:50. doi: 10.1186/s12864-016-3470-z

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — tRNA positional alignment distribution. Color indicates the tRNA by anticodon type. The x-axis denotes the position of tRNA from 0 to the end of tRNA. The y-axis denotes the cumulative read fractions. Visible quantity difference can be seen in the tRNA type detected by different kits. This suggests that there are selection bias of tRNA in by the kits