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. 2017 Jan 7;17:35. doi: 10.1186/s12885-016-2998-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Aberrant expression of CD34, SMA, and GS in cases with sinusoidal obstruction syndrome were used to calculate the CSI score. a In case 12, the immunohistochemistry for CD34 showed confluent sinusoidal staining extending from the periportal to midzonal and centrizonal areas (×100). This is an example of a immunohistochemistry grade 3 for CD34. b Immunohistochemical stain for SMA in the same case showed confluent subsinusoidal staining in multiple areas (×200), which is also an example of a grade 3 staining intensity. c Immunohistochemistry conducted on case 14 showed GS expression adjacent to an obliterated sublobular vein (arrow) that was expanded (×100) compared to the normal perivenular GS staining pattern and was also give an immunohistochemistry score of 3. d In Case 19, aberrant confluent GS expression is seen in midzonal and periportal areas; another example of a grade 3 pattern of staining (asterisks, central veins; arrow, portal tract; ×100)