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. 2017 Jan 7;18:10. doi: 10.1186/s12882-016-0415-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Survival analysis of the cecal ligation and puncture (CLP) model used in the study (a) and time-course of serum neutrophil gelatinase associated lipocalin (sNGAL) and serum creatinine (Scr) (b) were demonstrated. The representative pictures of the immunohistochemistry of mouse kidney after 6 h of CLP sepsis surgery for NGAL (c) and activating transcriptional factor 3 (ATF3) (d). NGAL and ATF3 were demonstrated in cytoplasm/brush border and nuclei of cortical renal tubules cells, respectively, and both parameters could not be detected in kidney of sham surgery (data not showed). Additionally, activated caspase-3 was also analyzed in kidney sample at 6 h of CLP and sham surgery (e). The comparison of Caspase-3 Western Blot density between kidney of sham and CLP group measured from C-DiGit® Blot Scanner was also showed (f)