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. 2017 Jan 7;36:7. doi: 10.1186/s13046-016-0481-1

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — PFK15 impairs the formation and function of invadopodia in Cal27 cells. a The extracellular matrix (ECM) degradation ability of Cal27 cells after PFK15 treatment were measured by seeding tumor cells on the Alexa568-gelatin coated coverslips. b PFK15 impairs the formation of invadopodia inCal27 cells, which were detected by co-localisation of cortactin, F-actin and the black holes on the Alexa568-gelatin coated coverslips. c The quantitative data of the degraded matrix on the Alexa568-gelatin coated coverslips. d The quantitative data of the invadopodia formation per Cal27 cells. e The localisation of PFKFB3 in the invadopodia of Cal27 cells. Mean ± S.E.M.; ***P < 0.001