Fig. 6.

Expression of dominant negative AMPKα or down-regulation of AMPKα attenuates CPT-induced inhibition of mTORC1 signaling. Serum-starved Rh30 cells respectively expressing ad-GFP, and ad-AMPK-DN (a), or sh-AMPK and sh-GFP (b), were firstly exposed to CPT (10 μmol/L) for 2 h, then stimulated with IGF-1 (10 ng/mL) for 1 h, followed by western blotting assay for proteins expression of HA, p-mTOR(2448), mTOR, p-S6K1, S6K1, 4E-BP1, p-4E-BP1(T70). And β-tubulin was a control