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. 2017 Jan 7;17:12. doi: 10.1186/s12866-016-0920-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Usage of Pyviko in stand-alone scripts and web interface. a Schematic of sample target (Y) and overlapping (X) genes used in code examples. b Minimal Python commands for generating gene knockouts. In this interactive example, “>>>” denotes input into a Python interpreter and output is displayed directly below the input on an unindented line. c Pyviko input interface [14] including sample gene sequences. d Results of analysis for input shown in (c)