Fig. 3.

Modulation of phospho-Akt and gemcitabine determinants. a Effect of 24-h exposure to gemcitabine, perifosine or their combination, at IC₅₀ values, on the expression of phospho-Akt, normalized to the expression of total Akt, as determined by ELISA. b Expression of gemcitabine key determinants in LPC028 (left panel) and LPC006 (right panel) cells treated with perifosine at IC₅₀ versus untreated cells, as determined by qRT-PCR. Columns mean values obtained from three independent experiments, bars, SEM. Dashed line, values in untreated samples (Control). *Significantly different from controls