Fig. 4.

Effects of perifosine, gemcitabine and their combination on PDAC cells migration and invasion. a Results of wound-healing assay in LPC028 and LPC006 cells exposed to perifosine, gemcitabine or to their combination, at IC50 values for 24 h. b Results of invasion studies in the PDAC cells exposed for 24 h to perifosine, gemcitabine, or to their combination, at IC₅₀ values (insert: representative pictures of LPC028 cells at 24 h, original magnification ×40). c Modulation of E-cadherin mRNA levels in LPC028, LPC006, PANC-1, and CFPAC-1 cells after 24-h exposure to perifosine, gemcitabine, or to their combination, at IC₅₀ values, as determined by qRT-PCR. Columns or points mean values obtained from three independent experiments; bars, SEM. *Significantly different from controls